Review




Structured Review

Promega 3x mtase-glo reagent
3x Mtase Glo Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtase-glo+reagent/3x+mtase+glo+reagent/pm40146197-484-21-24
Average 90 stars, based on 1 article reviews
3x mtase-glo reagent - by Bioz Stars, 2026-10
90/100 stars

Images

Related Articles

Functional Assay:

Article Title: AI-guided pipeline for protein–protein interaction drug discovery identifies a SARS-CoV-2 inhibitor
Article Snippet: After incubation, 2 μl of 5× MTase-Glo Reagent (Promega) was added to wells, plates were shaken for 1 min at 1000 rpm and further incubated for 30 min at RT.

Article Title: Targeting PRMT1-mediated SRSF1 methylation to suppress oncogenic exon inclusion events and breast tumorigenesis.
Article Snippet: MTase-Glo Assay (Promega V7601) was performed by adding the MTase-Glo Reagent to convert SAH to ADP, which was further converted to ATP by adding the MTase-Glo detection solution.

Article Title: From DNA-Encoded Library Screening to AM-9747 : An MTA-Cooperative PRMT5 Inhibitor with Potent Oral In Vivo Efficacy.
Article Snippet: After a 30 min incubation at RT, 8 μL of MTase-Glo Detection Solution (Promega) was added and the plate was incubated at RT for an additional 30 min.

Article Title: A sulfonamide chalcone inhibited dengue virus with a potential target at the SAM-binding site of viral methyltransferase.
Article Snippet: Dengue infection is a global health problem as climate change facilitates the spread of mosquito vectors.. Infected patients could progress to severe plasma leakage and hemorrhagic shock, where current standard treatment remains supportive.. Previous reports suggested that several flavonoid derivatives inhibited mosquito-borne flaviviruses.

Article Title: Lichen-Derived Diffractaic Acid Inhibited Dengue Virus Replication in a Cell-Based System.
Article Snippet: Then, 10 μL of the enzyme complex (100 μg/mL MTase, 1.25 μM capped RNA substrate, and 10× MTase-Glo reagent (Promega, Madison, WI, USA) in 4× reaction buffer (80 mM Tris–HCl pH 8.0, 200 mM NaCl, 4 mM EDTA, 12 mM MgCl2, 0.4 mg/mL BSA, and 4 mM DTT)) was mixed with 5 μL of various concentrations of diffractaic acid and incubated at room temperature for 10 min.

Article Title: Development of BacMam Induced Hepatitis E Virus Replication Model in Hepatoma Cells to Study the Polyprotein Processing
Article Snippet: To check if methyltransferase is functional in the cells, an enzyme assay was performed using MTase-Glo reagent (Promega), mentioned in Section “Materials and Methods.” It was found that methyltransferase activity increased with an increase in the enzyme concentration as compared to mock (uninfected cell) lysate ( ).

Article Title: From DNA-Encoded Library Screening to AM-9747 : An MTA-Cooperative PRMT5 Inhibitor with Potent Oral In Vivo Efficacy.
Article Snippet: The reaction was allowed to proceed for 20 h at RT and was then terminated by the addition of 1 μL of 9X MTase-Glo Reagent (Promega).

Enzymatic Assay:

Article Title: AI-guided pipeline for protein–protein interaction drug discovery identifies a SARS-CoV-2 inhibitor
Article Snippet: After incubation, 2 μl of 5× MTase-Glo Reagent (Promega) was added to wells, plates were shaken for 1 min at 1000 rpm and further incubated for 30 min at RT.

Article Title: Targeting PRMT1-mediated SRSF1 methylation to suppress oncogenic exon inclusion events and breast tumorigenesis.
Article Snippet: MTase-Glo Assay (Promega V7601) was performed by adding the MTase-Glo Reagent to convert SAH to ADP, which was further converted to ATP by adding the MTase-Glo detection solution.

Article Title: From DNA-Encoded Library Screening to AM-9747 : An MTA-Cooperative PRMT5 Inhibitor with Potent Oral In Vivo Efficacy.
Article Snippet: After a 30 min incubation at RT, 8 μL of MTase-Glo Detection Solution (Promega) was added and the plate was incubated at RT for an additional 30 min.

Article Title: A sulfonamide chalcone inhibited dengue virus with a potential target at the SAM-binding site of viral methyltransferase.
Article Snippet: Dengue infection is a global health problem as climate change facilitates the spread of mosquito vectors.. Infected patients could progress to severe plasma leakage and hemorrhagic shock, where current standard treatment remains supportive.. Previous reports suggested that several flavonoid derivatives inhibited mosquito-borne flaviviruses.

Article Title: Lichen-Derived Diffractaic Acid Inhibited Dengue Virus Replication in a Cell-Based System.
Article Snippet: Then, 10 μL of the enzyme complex (100 μg/mL MTase, 1.25 μM capped RNA substrate, and 10× MTase-Glo reagent (Promega, Madison, WI, USA) in 4× reaction buffer (80 mM Tris–HCl pH 8.0, 200 mM NaCl, 4 mM EDTA, 12 mM MgCl2, 0.4 mg/mL BSA, and 4 mM DTT)) was mixed with 5 μL of various concentrations of diffractaic acid and incubated at room temperature for 10 min.

Article Title: Development of BacMam Induced Hepatitis E Virus Replication Model in Hepatoma Cells to Study the Polyprotein Processing
Article Snippet: To check if methyltransferase is functional in the cells, an enzyme assay was performed using MTase-Glo reagent (Promega), mentioned in Section “Materials and Methods.” It was found that methyltransferase activity increased with an increase in the enzyme concentration as compared to mock (uninfected cell) lysate ( ).

Article Title: From DNA-Encoded Library Screening to AM-9747 : An MTA-Cooperative PRMT5 Inhibitor with Potent Oral In Vivo Efficacy.
Article Snippet: The reaction was allowed to proceed for 20 h at RT and was then terminated by the addition of 1 μL of 9X MTase-Glo Reagent (Promega).

Activity Assay:

Article Title: AI-guided pipeline for protein–protein interaction drug discovery identifies a SARS-CoV-2 inhibitor
Article Snippet: After incubation, 2 μl of 5× MTase-Glo Reagent (Promega) was added to wells, plates were shaken for 1 min at 1000 rpm and further incubated for 30 min at RT.

Article Title: Targeting PRMT1-mediated SRSF1 methylation to suppress oncogenic exon inclusion events and breast tumorigenesis.
Article Snippet: MTase-Glo Assay (Promega V7601) was performed by adding the MTase-Glo Reagent to convert SAH to ADP, which was further converted to ATP by adding the MTase-Glo detection solution.

Article Title: From DNA-Encoded Library Screening to AM-9747 : An MTA-Cooperative PRMT5 Inhibitor with Potent Oral In Vivo Efficacy.
Article Snippet: After a 30 min incubation at RT, 8 μL of MTase-Glo Detection Solution (Promega) was added and the plate was incubated at RT for an additional 30 min.

Article Title: A sulfonamide chalcone inhibited dengue virus with a potential target at the SAM-binding site of viral methyltransferase.
Article Snippet: Dengue infection is a global health problem as climate change facilitates the spread of mosquito vectors.. Infected patients could progress to severe plasma leakage and hemorrhagic shock, where current standard treatment remains supportive.. Previous reports suggested that several flavonoid derivatives inhibited mosquito-borne flaviviruses.

Article Title: Lichen-Derived Diffractaic Acid Inhibited Dengue Virus Replication in a Cell-Based System.
Article Snippet: Then, 10 μL of the enzyme complex (100 μg/mL MTase, 1.25 μM capped RNA substrate, and 10× MTase-Glo reagent (Promega, Madison, WI, USA) in 4× reaction buffer (80 mM Tris–HCl pH 8.0, 200 mM NaCl, 4 mM EDTA, 12 mM MgCl2, 0.4 mg/mL BSA, and 4 mM DTT)) was mixed with 5 μL of various concentrations of diffractaic acid and incubated at room temperature for 10 min.

Article Title: Development of BacMam Induced Hepatitis E Virus Replication Model in Hepatoma Cells to Study the Polyprotein Processing
Article Snippet: To check if methyltransferase is functional in the cells, an enzyme assay was performed using MTase-Glo reagent (Promega), mentioned in Section “Materials and Methods.” It was found that methyltransferase activity increased with an increase in the enzyme concentration as compared to mock (uninfected cell) lysate ( ).

Article Title: From DNA-Encoded Library Screening to AM-9747 : An MTA-Cooperative PRMT5 Inhibitor with Potent Oral In Vivo Efficacy.
Article Snippet: The reaction was allowed to proceed for 20 h at RT and was then terminated by the addition of 1 μL of 9X MTase-Glo Reagent (Promega).

Concentration Assay:

Article Title: AI-guided pipeline for protein–protein interaction drug discovery identifies a SARS-CoV-2 inhibitor
Article Snippet: After incubation, 2 μl of 5× MTase-Glo Reagent (Promega) was added to wells, plates were shaken for 1 min at 1000 rpm and further incubated for 30 min at RT.

Article Title: Targeting PRMT1-mediated SRSF1 methylation to suppress oncogenic exon inclusion events and breast tumorigenesis.
Article Snippet: MTase-Glo Assay (Promega V7601) was performed by adding the MTase-Glo Reagent to convert SAH to ADP, which was further converted to ATP by adding the MTase-Glo detection solution.

Article Title: From DNA-Encoded Library Screening to AM-9747 : An MTA-Cooperative PRMT5 Inhibitor with Potent Oral In Vivo Efficacy.
Article Snippet: After a 30 min incubation at RT, 8 μL of MTase-Glo Detection Solution (Promega) was added and the plate was incubated at RT for an additional 30 min.

Article Title: A sulfonamide chalcone inhibited dengue virus with a potential target at the SAM-binding site of viral methyltransferase.
Article Snippet: Dengue infection is a global health problem as climate change facilitates the spread of mosquito vectors.. Infected patients could progress to severe plasma leakage and hemorrhagic shock, where current standard treatment remains supportive.. Previous reports suggested that several flavonoid derivatives inhibited mosquito-borne flaviviruses.

Article Title: Lichen-Derived Diffractaic Acid Inhibited Dengue Virus Replication in a Cell-Based System.
Article Snippet: Then, 10 μL of the enzyme complex (100 μg/mL MTase, 1.25 μM capped RNA substrate, and 10× MTase-Glo reagent (Promega, Madison, WI, USA) in 4× reaction buffer (80 mM Tris–HCl pH 8.0, 200 mM NaCl, 4 mM EDTA, 12 mM MgCl2, 0.4 mg/mL BSA, and 4 mM DTT)) was mixed with 5 μL of various concentrations of diffractaic acid and incubated at room temperature for 10 min.

Article Title: Development of BacMam Induced Hepatitis E Virus Replication Model in Hepatoma Cells to Study the Polyprotein Processing
Article Snippet: To check if methyltransferase is functional in the cells, an enzyme assay was performed using MTase-Glo reagent (Promega), mentioned in Section “Materials and Methods.” It was found that methyltransferase activity increased with an increase in the enzyme concentration as compared to mock (uninfected cell) lysate ( ).

Article Title: From DNA-Encoded Library Screening to AM-9747 : An MTA-Cooperative PRMT5 Inhibitor with Potent Oral In Vivo Efficacy.
Article Snippet: The reaction was allowed to proceed for 20 h at RT and was then terminated by the addition of 1 μL of 9X MTase-Glo Reagent (Promega).



Similar Products

90
Promega 3x mtase-glo reagent
3x Mtase Glo Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtase-glo+reagent/3x+mtase+glo+reagent/pm40146197-484-21-24
Average 90 stars, based on 1 article reviews
3x mtase-glo reagent - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Promega mtase-glo reagent
Mtase Glo Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtase-glo+reagent/mtase+glo+reagent/pm40102181-489-22-25
Average 90 stars, based on 1 article reviews
mtase-glo reagent - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Promega mtase glo reagent
( A ) Diagram of enzymatic reaction carried ofout by RNMT. The N7 nitrogen on the cap and the methyl group are highlighted in red. ( B ) The cap structure minus RNA as used in the present paperstudy. ( C ) GMP-PnP as used as a cap surrogate in the crystallography experiments and ( D ) Sinefungin, a <t>non-selective</t> <t>methyltransferase</t> inhibitor acting as a SAM mimetic. Abbreviations: GMP-PnP, guanosine 5’-[β,γ-imido]triphosphate; RNMT, RNA guanine-7 methyltransferase; SAM, S-adenosylmethionine.
Mtase Glo Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtase-glo+reagent/mtase+glo+reagent/pmc12133303-56-4-7
Average 90 stars, based on 1 article reviews
mtase glo reagent - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Promega 5× concentrated mtase-glo™ reagent
( A ) Diagram of enzymatic reaction carried ofout by RNMT. The N7 nitrogen on the cap and the methyl group are highlighted in red. ( B ) The cap structure minus RNA as used in the present paperstudy. ( C ) GMP-PnP as used as a cap surrogate in the crystallography experiments and ( D ) Sinefungin, a <t>non-selective</t> <t>methyltransferase</t> inhibitor acting as a SAM mimetic. Abbreviations: GMP-PnP, guanosine 5’-[β,γ-imido]triphosphate; RNMT, RNA guanine-7 methyltransferase; SAM, S-adenosylmethionine.
5× Concentrated Mtase Glo™ Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtase-glo+reagent/5%C3%97mtase+glo++reagent/us11939344-305-4-7
Average 90 stars, based on 1 article reviews
5× concentrated mtase-glo™ reagent - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


( A ) Diagram of enzymatic reaction carried ofout by RNMT. The N7 nitrogen on the cap and the methyl group are highlighted in red. ( B ) The cap structure minus RNA as used in the present paperstudy. ( C ) GMP-PnP as used as a cap surrogate in the crystallography experiments and ( D ) Sinefungin, a non-selective methyltransferase inhibitor acting as a SAM mimetic. Abbreviations: GMP-PnP, guanosine 5’-[β,γ-imido]triphosphate; RNMT, RNA guanine-7 methyltransferase; SAM, S-adenosylmethionine.

Journal: Biochemical Journal

Article Title: Characterisation of RNA guanine-7 methyltransferase (RNMT) using a small molecule approach

doi: 10.1042/BCJ20240608

Figure Lengend Snippet: ( A ) Diagram of enzymatic reaction carried ofout by RNMT. The N7 nitrogen on the cap and the methyl group are highlighted in red. ( B ) The cap structure minus RNA as used in the present paperstudy. ( C ) GMP-PnP as used as a cap surrogate in the crystallography experiments and ( D ) Sinefungin, a non-selective methyltransferase inhibitor acting as a SAM mimetic. Abbreviations: GMP-PnP, guanosine 5’-[β,γ-imido]triphosphate; RNMT, RNA guanine-7 methyltransferase; SAM, S-adenosylmethionine.

Article Snippet: The first utilises the MTase Glo reagent (Promega) to convert the product of the methyltransferase assay into a bioluminescent signal [ ] through coupling enzymes.

Techniques:

Both measure SAH. The MTase-Glo™ assay uses coupling enzymes to convert SAH to a light readout. The RapidFire™ mass spectrometry assay measures SAH levels directly. Abbreviation: SAH, S-adenosylhomocysteine.

Journal: Biochemical Journal

Article Title: Characterisation of RNA guanine-7 methyltransferase (RNMT) using a small molecule approach

doi: 10.1042/BCJ20240608

Figure Lengend Snippet: Both measure SAH. The MTase-Glo™ assay uses coupling enzymes to convert SAH to a light readout. The RapidFire™ mass spectrometry assay measures SAH levels directly. Abbreviation: SAH, S-adenosylhomocysteine.

Article Snippet: The first utilises the MTase Glo reagent (Promega) to convert the product of the methyltransferase assay into a bioluminescent signal [ ] through coupling enzymes.

Techniques: Glo Assay, Mass Spectrometry